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questions :hydrophobic interaction chromatographic?
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[Visitor (58.214.*.*)]answers [Chinese ]Time :2020-07-25
This paper reports a method of purifying a polypeptide comprising a histidine tag, the method comprising the following steps: i) applying a solution comprising a polypeptide having a histidine tag to a hydrophobic interaction chromatography material, and ii) using an imidazole or imidazole The solution of the derivative recovers the polypeptide containing the histidine tag, and thereby purifies the polypeptide containing the histidine tag, wherein the polypeptide-containing solution applied to the hydrophobic interaction chromatography material does not contain imidazole or imidazole derivatives, and is adsorbed to the hydrophobic interaction The peptide of the action chromatography material is recovered with a solution containing imidazole or imidazole derivatives.

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